Transcription initiation is the most regulated stage of the transcription cycle that directly affects the overall level of gene expression. In bacteria, recognition of promoters by RNA polymerase is controlled by the a subunit. Recent structural analysis of RNA polymerase and its complexes with nucleic acids reveals that distinct structural domains of the RNA polymerase core enzyme are close to or contact promoter DNA, suggesting that they too may participate in promoter recognition and control promoter complex stability and activity. In this review, the data from our laboratory that highlight the role of RNA polymerase core-enzyme in promoter recognition is discussed.
|Number of pages||10|
|Publication status||Published - May 2007|