Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium

Yoshimasa Oyama, Colleen M. Bartman, Stephanie Bonney, J. Scott Lee, Lori A. Walker, Jun Han, Christoph H. Borchers, Peter M. Buttrick, Carol M. Aherne, Nathan Clendenen, Sean P. Colgan, Tobias Eckle

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Consistent daylight oscillations and abundant oxygen availability are fundamental to human health. Here, we investigate the intersection between light-sensing (Period 2 [PER2]) and oxygen-sensing (hypoxia-inducible factor [HIF1A]) pathways in cellular adaptation to myocardial ischemia. We demonstrate that intense light is cardioprotective via circadian PER2 amplitude enhancement, mimicking hypoxia-elicited adenosine- and HIF1A-metabolic adaptation to myocardial ischemia under normoxic conditions. Whole-genome array from intense light-exposed wild-type or Per2−/− mice and myocardial ischemia in endothelial-specific PER2-deficient mice uncover a critical role for intense light in maintaining endothelial barrier function via light-enhanced HIF1A transcription. A proteomics screen in human endothelia reveals a dominant role for PER2 in metabolic reprogramming to hypoxia via mitochondrial translocation, tricarboxylic acid (TCA) cycle enzyme activity regulation, and HIF1A transcriptional adaption to hypoxia. Translational investigation of intense light in human subjects identifies similar PER2 mechanisms, implicating the use of intense light for the treatment of cardiovascular disease. Oyama et al. investigate the mechanisms that underlie intense light-mediated protection from myocardial ischemia and find that intense light increases the circadian amplitude of PER2, which preconditions the myocardium via adenosine and HIF1A transcriptional reprogramming of the endothelium before an ischemic event.

Original languageEnglish
Pages (from-to)1471-1484.e11
JournalCell Reports
Volume28
Issue number6
DOIs
Publication statusPublished - 6 Aug 2019
Externally publishedYes

Keywords

  • circadian
  • Claudin-1
  • COX4
  • endothelium
  • HIF1A
  • human subjects
  • intense light
  • metabolism
  • myocardial ischemia and reperfusion injury
  • PER2
  • PER2 floxed
  • tissue-specific mice
  • wheel running

Fingerprint

Dive into the research topics of 'Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium'. Together they form a unique fingerprint.

Cite this