Infrared fluorescent protein iRFP as an acceptor for resonance excitation energy transfer

O. A. Zlobovskaya, K. S. Sarkisyan, K. A. Lukyanov

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The possibility of bacteriochrome-based infrared fluorescent protein iRFP use as an acceptor for the Förster resonance energy transfer (FRET) was investigated. GFP-like far-red fluorescent proteins mKate2, eqFP650, and eqFP670 were used as energy donors. Bacterial expression vectors encoding donor and acceptor proteins joined by a heptadecapeptide linker were constructed with the goal to test FRET. The efficiency of FRET was estimated in vitro for the isolated proteins from the increase of the donor emission following cleavage of the linker. Among the three tested constructs the eqFP650-iRFP pair demonstrated the most efficient energy transfer (approximately 30%).

Original languageEnglish
Pages (from-to)266-270
Number of pages5
JournalRussian Journal of Bioorganic Chemistry
Volume41
Issue number3
DOIs
Publication statusPublished - 29 May 2015
Externally publishedYes

Keywords

  • fluorescent protein
  • FRET
  • genetically encoded sensor
  • GFP
  • iRFP

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