Increased depth and breadth of plasma protein quantitation via two-dimensional liquid chromatography/multiple reaction monitoring-mass spectrometry with labeled peptide standards

Andrew J. Percy, Juncong Yang, Andrew G. Chambers, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Absolute quantitative strategies are emerging as a powerful and preferable means of deriving concentrations in biological samples for systems biology applications. Method development is driven by the need to establish new—and validate current—protein biomarkers of high-to-low abundance for clinical utility. In this chapter, we describe a methodology involving two-dimensional (2D) reversed-phase liquid chromatography (RPLC), operated under alkaline and acidic pH conditions, combined with multiple reaction monitoring (MRM)-mass spectrometry (MS) (also called selected reaction monitoring (SRM)-MS) and a complex mixture of stable isotope-labeled standard (SIS) peptides, to quantify a broad and diverse panel of 253 proteins in human blood plasma. The quantitation range spans 8 orders of magnitude—from 15 mg/mL (for vitamin D-binding protein) to 450 pg/mL (for protein S100-B)—and includes 31 lowabundance proteins (defined as being 10 ng/mL) of potential disease relevance. The method is designed to assess candidates at the discovery and/or verification phases of the biomarker pipeline and can be adapted to examine smaller or alternate panels of proteins for higher sample throughput. Also detailed here is the application of our recently developed software tool—Qualis-SIS—for protein quantitation (via regression analysis of standard curves) and quality assessment of the resulting data. Overall, this chapter provides the blueprint for the replication of this quantitative proteomic method by proteomic scientists of all skill levels.

Original languageEnglish
Pages (from-to)1-21
Number of pages21
JournalMethods in Molecular Biology
Volume1410
DOIs
Publication statusPublished - 1 Feb 2016
Externally publishedYes

Keywords

  • Multidimensional liquid chromatography
  • Multiple reaction monitoring
  • Plasma
  • Protein
  • Proteomics
  • Quantitation
  • Sensitive
  • Stable isotope-labeled standard

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