Improved protein identification through the use of unstained gels

David R. Loiselle, William R. Thelin, Carol E. Parker, Nedyalka N. Dicheva, Barry A. Kesner, Viorel Mocanu, Frank Wang, Sharon L. Milgram, Maria R.Esteban Warren, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silver-staining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data.

Original languageEnglish
Pages (from-to)992-997
Number of pages6
JournalJournal of Proteome Research
Volume4
Issue number3
DOIs
Publication statusPublished - May 2005
Externally publishedYes

Keywords

  • Automation
  • Immunoprecipitation
  • MALDI-MS
  • Page
  • Protein identification
  • Robotics
  • Silver staining
  • Unstained gels

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