The identification of protein-protein interactions both in vitro and in vivo is discussed. The photochemical crosslinking and mass spectrometric techniques are used for the purpose. A targeted proteomic approach for studying protein-protein interactions is developed. The approach offers several potential advantages for identifying components of protein complexes. These include trapping weak or transient interactions, temporal control of crosslinking for identification of partners in a dynamic system, elimination of false positives by using crosslinker-specific markers and localization of binding sites on interacting proteins.
|Number of pages||2|
|Publication status||Published - 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2 Jun 2002 → 6 Jun 2002
|Conference||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||2/06/02 → 6/06/02|