Identification of protein-protein interactions both in vitro and in vivo using photochemical crosslinking and mass spectrometry

Mark C. Hall, Ekaterina Bassett, Nana Nikolaishvili-Feinberg, Stephen G. Chaney, Marila Cordeiro-Stone, Christoph H. Borchers

Research output: Contribution to conferencePaperpeer-review

Abstract

The identification of protein-protein interactions both in vitro and in vivo is discussed. The photochemical crosslinking and mass spectrometric techniques are used for the purpose. A targeted proteomic approach for studying protein-protein interactions is developed. The approach offers several potential advantages for identifying components of protein complexes. These include trapping weak or transient interactions, temporal control of crosslinking for identification of partners in a dynamic system, elimination of false positives by using crosslinker-specific markers and localization of binding sites on interacting proteins.

Original languageEnglish
Pages249-250
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2/06/026/06/02

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