Identification of immediate early genes in the nervous system of snail helix lucorum

Chuan Xu, Qian Li, Olga Efimova, Xi Jiang, Marina Petrova, Alia K. Vinarskaya, Peter Kolosov, Nikolay Aseyev, Kira Koshkareva, Victor N. Ierusalimsky, Pavel M. Balaban, Philipp Khaitovich

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Immediate early genes (IEGs) are useful markers of neuronal activation and essential components of neuronal response. While studies of gastropods have provided many insights into the basic learning and memory mechanisms, the genome-wide assessment of IEGs has been mainly restricted to vertebrates. In this study, we identified IEGs in the terrestrial snail Helix lucorum. In the absence of the genome, we conducted de novo transcriptome assembly using reads with short and intermediate lengths cumulatively covering more than 98 billion nucleotides. Based on this assembly, we identified 37 proteins corresponding to contigs differentially expressed (DE) in either the parietal ganglia (PaG) or two giant interneurons located within the PaG of the snail in response to the neuronal stimulation. These proteins included homologues of well-known mammalian IEGs, such as c-jun/jund, C/EBP, c-fos/fosl2, and Egr1, as well as homologues of genes not yet implicated in the neuronal response.

    Original languageEnglish
    Article numbere0416-18.2019
    JournaleNeuro
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 1 May 2019

    Keywords

    • Helix lucorum
    • Immediate early genes
    • Nervous system
    • Snail

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