Identification and characterization of andalusicin: N-terminally dimethylated class III lantibiotic from Bacillus thuringiensis sv. andalousiensis

Anastasiia Grigoreva, Julia Andreeva, Dmitry Bikmetov, Anastasiia Rusanova, Marina Serebryakova, Andrea Hernandez Garcia, Darya Slonova, Satish K. Nair, Guy Lippens, Konstantin Severinov, Svetlana Dubiley

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Lanthipeptides, ribosomally synthesized and post-translationally modified peptides (RiPPs), can be divided into five classes based on their structures and biosynthetic pathways. Class I and II lanthipeptides have been well characterized, whereas less is known about members of the other three classes. Here, we describe a new family of class III lanthipeptides from Firmicutes. Members of the family are distinguished by the presence of a single carboxy-terminal labionin. We identified and characterized andalusicin, a representative of this family. Andalusicin bears two methyl groups at the α-amino terminus, a post-translational modification that has not previously been identified in class III lanthipeptides. Mature andalusicin A shows bioactivity against various Gram-positive bacteria, an activity that is highly dependent on the α-N dimethylation.

Original languageEnglish
Article number102480
JournaliScience
Volume24
Issue number5
DOIs
Publication statusPublished - 21 May 2021

Keywords

  • Biochemistry
  • Biosynthesis
  • Chemistry
  • Medical biochemistry

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