Hydroxyethylstarch in cryopreservation - Mechanisms, benefits and problems

A. Stolzing, Y. Naaldijk, V. Fedorova, S. Sethe

Research output: Contribution to journalReview articlepeer-review

62 Citations (Scopus)


As the progress of regenerative medicine places ever greater attention on cryopreservation of (stem) cells, tried and tested cryopreservation solutions deserve a second look. This article discusses the use of hydroxyethyl starch (HES) as a cryoprotectant. Charting carefully the recorded uses of HES as a cryoprotectant, in parallel to its further clinical use, indicates that some HES subtypes are a useful supplement to dimethysulfoxide (DMSO) in cryopreservation. However, we suggest that the most common admixture ratio of HES and DMSO in cryoprotectant solutions has been established by historical happenstance and requires further investigation and optimization.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalTransfusion and Apheresis Science
Issue number2
Publication statusPublished - Apr 2012
Externally publishedYes


  • Cryopreservation
  • Dimethysulfoxide
  • Hydroxyethyl starch
  • Stem cells


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