Human Short Peptidoglycan Recognition Protein PGLYRP1/Tag-7/PGRP-S Inhibits Listeria monocytogenes Intracellular Survival in Macrophages

Darya Slonova, Alexandra Posvyatenko, Alexey Kibardin, Elena Sysolyatina, Elena Lyssuk, Svetlana Ermolaeva, Sergei Obydennyi, Nikolay Gnuchev, Georgii Georgiev, Konstantin Severinov, Sergey Larin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

PGLYRP1/Tag-7/PGRP-S is one of mammalian peptidoglycan recognition proteins (PGRPs). Here, we demonstrate that human recombinant PGLYRP1/Tag-7/PGRP-S potentiates the response of murine macrophage-like ANA-1 cells and human macrophages to facultative intracellular pathogen Listeria monocytogenes. PGLYRP1/Tag-7/PGRP-S binds to the surface of L. monocytogenes and other bacterial cells but has no effect on their growth in culture. While PGLYRP1/Tag-7/PGRP-S treatment modestly enhanced phagocytosis of bacteria by ANA-1 cells, the intracellular survival of PGLYRP1/Tag-7/PGRP-S treated L. monocytogenes was strongly inhibited 2 h after internalization. PGLYRP1/Tag-7/PGRP-S treatment of bacteria boosted oxidative burst induction and increased the level of proinflammatory cytokine IL-6 produced by ANA-1, however, these effects happened too late to be responsible for decreased intracellular survival of bacteria. Our results thus suggest that PGLYRP1/Tag-7/PGRP-S acts as a molecular sensor for detection of L. monocytogenes infection of mammalian cells that leads to increased killing through a mechanism(s) that remains to be defined.

Original languageEnglish
Article number582803
JournalFrontiers in Cellular and Infection Microbiology
Volume10
DOIs
Publication statusPublished - 23 Dec 2020

Keywords

  • innate immunity
  • Listeria monocytogenes
  • peptidoglycan recognition protein
  • peptidoglycan recognition protein-S
  • PGLYRP1 protein
  • phagocytosis
  • Tag-7

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