How to predict product yield in kinetically controlled enzymatic peptide synthesis

Mikhail Yu Gololobov, Elena V. Kozlova, Ivan L. Borisov, Ute Schellenberger, Volker Schellenberger, Hans‐Dieter ‐D Jakubke

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters α and β for papain‐catalyzed synthesis of Mal‐L‐Phe‐L‐Ala‐LLeuNH2 from Mal‐L‐Phe‐L‐AlaOMe and L‐LeuNH2. The experimental results demonstrate that this theory adequately describes the real process. © 1992 John Wiley & Sons, Inc.

    Original languageEnglish
    Pages (from-to)432-436
    Number of pages5
    JournalBiotechnology and Bioengineering
    Volume40
    Issue number3
    DOIs
    Publication statusPublished - Jul 1992

    Keywords

    • papain‐catalyzed peptide synthesis
    • progress curve kinetics
    • theory of enzymatic peptide synthesis

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