The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters α and β for papain‐catalyzed synthesis of Mal‐L‐Phe‐L‐Ala‐LLeuNH2 from Mal‐L‐Phe‐L‐AlaOMe and L‐LeuNH2. The experimental results demonstrate that this theory adequately describes the real process. © 1992 John Wiley & Sons, Inc.
- papain‐catalyzed peptide synthesis
- progress curve kinetics
- theory of enzymatic peptide synthesis