How can elongation factors EF-G and EF-Tu discriminate the functional state of the ribosome using the same binding site?

Petr V. Sergiev, Alexey A. Bogdanov, Olga A. Dontsova

Research output: Contribution to journalShort surveypeer-review

26 Citations (Scopus)

Abstract

Elongation factors EF-G and EF-Tu are structural homologues and share near-identical binding sites on the ribosome, which encompass the GTPase-associated centre (GAC) and the sarcin-ricin loop (SRL). The SRL is fixed structure in the ribosome and contacts elongation factors in the vicinity of their GTP-binding site. In contrast, the GAC is mobile and we hypothesize that it interacts with the alpha helix D of the EF-Tu G-domain in the same way as with the alpha helix A of the G′-domain of EF-G. The mutual locations of these helices and GTP-binding sites in the structures of EF-Tu and EF-G are different. Thus, the orientation of the GAC relative to the SRL determines whether EF-G or EF-Tu will bind to the ribosome.

Original languageEnglish
Pages (from-to)5439-5442
Number of pages4
JournalFEBS Letters
Volume579
Issue number25
DOIs
Publication statusPublished - 24 Oct 2005
Externally publishedYes

Keywords

  • EF-G
  • EF-Tu
  • Elongation factors
  • GTPase-associated centre
  • Ribosome

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