Histological study of subcutaneous fat at NIR laser treatment of the rat skin in vivo

Yu Yanina, Yu I. Svenskaya, N. A. Navolokin, O. V. Matveeva, A. B. Bucharskaya, G. N. Maslyakova, D. A. Gorin, G. B. Sukhorukov, V. V. Tuchin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


The goal of this work is to quantify impact of in vivo photochemical treatment using indocyanine green (ICG) or encapsulated ICG and NIR laser irradiation through skin of rat with obesity by the follow up tissue sampling and histochemistry. After 1 hour elapsed since 1-min light exposure samples of rat skin with subcutaneous tissue of thickness of 1.5-2.5 mm were taken by surgery from rats within marked 4-zones of the skin site. For hematoxylin-eosin histological examination of excised tissue samples, fixation was carried out by 10%-formaldehyde solution. For ICG and encapsulated ICG subcutaneous injection and subsequent 1-min diode laser irradiation with power density of 8 W/cm2, different necrotic regions with lipolysis of subcutaneous fat were observed. The obtained data can be used for safe layerby- layer laser treatment of obesity and cellulite.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2015
PublisherOSA - The Optical Society
ISBN (Print)9781628417012
Publication statusPublished - 21 Jul 2014
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2015 - Munich, Germany
Duration: 21 Jun 201525 Jun 2015

Publication series

NameOptics InfoBase Conference Papers


ConferenceEuropean Conference on Biomedical Optics, ECBO 2015


  • Adipose tissue
  • Histological analysis
  • Laser irradiation
  • Necrosis
  • Photodynamic action


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