High-throughput immunoglobulin sequencing data analysis with the use of unique molecular identifiers

M. Y. Lebedin, M. A. Turchaninova, E. S. Egorov, O. V. Britanova, D. M. Chudakov

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)

Abstract

High-throughput sequencing made deep analysis of immune receptors repertoires possible. However, B cells' ability to accumulate hypermutations indistinguishable from PCR errors along whole variable domain and low quality of extended sequencing still impose a serious obstacle to analysis of full-length variable immunoglobulin sequence repertoires. In present review we demonstrate how molecular barcoding technique (unique molecular identifers, UMI) and bioinformatics analysis provide nearly error-free immunoglobulin repertoires profling of complex B-cell populations comprising minor hypermutated subvariants.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalImmunologiya
Volume38
Issue number1
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • Antibody repertoire
  • B cells
  • High-throughput sequencing
  • Humoral adaptive immunity
  • Molecular barcoding

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