High expression levels and nuclear localization of novel Danio rerio ncRNA transcribed from a genomic region containing repetitive elements

O. S. Shubernetskaya, D. A. Skvortsov, S. A. Evfratov, M. P. Rubtsova, E. V. Belova, O. S. Strelkova, V. D. Cherepaninets, O. A. Zhironkina, A. M. Olovnikov, M. E. Zvereva, I. I. Kireev, O. A. Dontsova

Research output: Contribution to journalArticlepeer-review

Abstract

Noncoding and repetitive sequences make up a large part of the genome of high eukaryotes, but the elucidation of their roles and mechanisms of action are poorly understood. In this work, we found that interstitial telomeric repeats in the genome of Danio rerio colocalize with repetitive elements, including hAT and EnSpm, which are widely represented in vertebrate genomes. The investigation of a genomic region containing two pairs of these repeats located in close proximity showed that the area is transcribed. RNA-dependent structures containing this sequence were identified in D. rerio fibroblast nuclei, which indicates the functional importance of genomic repetitive elements or their transcripts.

Original languageEnglish
Pages (from-to)563-572
Number of pages10
JournalMolecular Biology
Volume48
Issue number4
DOIs
Publication statusPublished - Jul 2014
Externally publishedYes

Keywords

  • ageing
  • chromatin organization
  • Danio rerio
  • fibroblasts
  • fluorescence in situ hybridization
  • interstitial telomeric repeats
  • noncoding RNA
  • repetitive elements

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