Green fluorescent protein with tryptophan-based chromophore stable at low pH

D. A. Gorbachev, K. S. Sarkisyan, A. S. Mishin, K. A. Lukyanov

Research output: Contribution to journalLetterpeer-review

1 Citation (Scopus)

Abstract

Using directed (substitution T203Y) and subsequent random mutagenesis of the monomeric cyan fluorescent protein mTurquoise2, we obtained a protein with a tryptophan-based chromophore that fluoresces in the green region of the spectrum (excitation maximum 482 nm, emission maximum 519 nm). Fluorescence of the new protein is highly stable in a wide range of pH (pKa 4.9), more stable than all monomeric green fluorescent proteins with a tyrosine-based chromophore.

Original languageEnglish
Pages (from-to)220-222
Number of pages3
JournalRussian Journal of Bioorganic Chemistry
Volume43
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Keywords

  • bathochromic shift
  • GFP
  • mutagenesis
  • pH-sensitivity

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