GFP-like chromoproteins as a source of far-red fluorescent proteins

Nadya G. Gurskaya, Arkady F. Fradkov, Alexey Terskikh, Mikhail V. Matz, Yulii A. Labas, Vladimir I. Martynov, Yurii G. Yanushevich, Konstantin A. Lukyanov, Sergey A. Lukyanov

Research output: Contribution to journalArticlepeer-review

233 Citations (Scopus)

Abstract

We have employed a new approach to generate novel fluorescent proteins (FPs) from red absorbing chromoproteins. An identical single amino acid substitution converted novel chromoproteins from the species Anthozoa (Heteractis crispa, Condylactis gigantea, and Goniopora tenuidens) into far-red FPs (emission λmax=615-640 nm). Moreover, coupled site-directed and random mutagenesis of the chromoprotein from H. crispa resulted in a unique far-red FP (HcRed) that exhibited bright emission at 645 nm. A clear red shift in fluorescence of HcRed, compared to drFP583 (by more than 60 nm), makes it an ideal additional color for multi-color labeling. Importantly, HcRed is excitable by 600 nm dye laser, thus promoting new detection channels for multi-color flow cytometry applications. In addition, we generated a dimeric mutant with similar maturation and spectral properties to tetrameric HcRed.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalFEBS Letters
Volume507
Issue number1
DOIs
Publication statusPublished - 19 Oct 2001
Externally publishedYes

Keywords

  • Anthozoa
  • drFP583
  • DsRed
  • Green fluorescent protein
  • Multi-color labeling

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