Genetically encoded sensors and fluorescence microscopy for anticancer research

Elena V. Zagaynova, Marina V. Shirmanova, Tatiana F. Sergeeva, Natalia V. Klementieva, Alexander S. Mishin, Alena I. Gavrina, Olga A. Zlobovskaya, Olga E. Furman, Varvara V. Dudenkova, Gregory S. Perelman, Maria M. Lukina, Konstantin A. Lukyanov

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Early response of cancer cells to chemical compounds and chemotherapeutic drugs were studied using novel fluorescence tools and microscopy techniques. We applied confocal microscopy, two-photon fluorescence lifetime imaging microscopy and super-resolution localization-based microscopy to assess structural and functional changes in cancer cells in vitro. The dynamics of energy metabolism, intracellular pH, caspase-3 activation during staurosporine-induced apoptosis as well as actin cytoskeleton rearrangements under chemotherapy were evaluated. We have showed that new genetically encoded sensors and advanced fluorescence microscopy methods provide an efficient way for multiparameter analysis of cell activities.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVII
EditorsKarsten Konig, Peter T. C. So, Ammasi Periasamy, Xiaoliang S. Xie
PublisherSPIE
ISBN (Electronic)9781510605794
DOIs
Publication statusPublished - 2017
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences XVII - San Francisco, United States
Duration: 29 Jan 201731 Jan 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10069
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XVII
Country/TerritoryUnited States
CitySan Francisco
Period29/01/1731/01/17

Keywords

  • actin cytoskeleton
  • caspase-3
  • FAD
  • fluorescence microscopy
  • intracellular pH
  • NAD(P)H

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