Genetically encoded intracellular sensors based on fluorescent proteins

E. A. Souslova, D. M. Chudakov

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Green fluorescent protein from Aequorea victoria and its many homologs are now widely used in basic and applied research. These genetically encoded fluorescent markers can detect localization of cell proteins and organelles in living cells and also cells and tissues in living organisms. Unique instruments and methods for studies of molecular biology of a cell and high throughput drug screenings are based on fluorescent proteins. This review deals with the most intensively evolving directions in this field, the development of genetically encoded sensors. Changes in their spectral properties are used for monitoring of cell enzyme activities or changes in concentrations of particular molecules.

Original languageEnglish
Pages (from-to)683-697
Number of pages15
JournalBiochemistry (Moscow)
Volume72
Issue number7
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

Keywords

  • Fluorescent proteins
  • FRET
  • Genetically encoded sensors
  • Mutant GFP variants

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