Genetic control of in vitro-organogenesis in recombinant inbred lines of sunflower (Helianthus annuus L.)

E. F. Berrios, L. Gentzbittel, G. Alibert, Y. Griveau, A. Bervillé, A. Sarrafi

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13 Citations (Scopus)


Ninety-three recombinant inbred lines (F8) of sunflower developed by the single-seed descent method from the cross 'PAC-2 x RHA-266' were used to screen their regenerability by organogenesis. The experiment was designed in randomized complete blocks with three replications. Each replication consisted of 10 Petri dishes with four explants. Cotyledons were excised from 2-day-old seedlings. Each cotyledon was divided into two pieces (four explants), which were incubated in solid regeneration medium consisting of full-strength Murashige and Skoog medium modified by adding hormones. A high genotypic variability for organogenesis parameters between genotypes was observed in this study. The difference between all recombinant F8 lines and their parents was not significant, showing that the 93 inbred lines used in this experiment are representative of the total possible recombinant lines from the cross 'PAC-2 x RHA-266'. These F8 lines were not consciously selected for any trait; therefore, they represent a random set of lines segregating for the organogenesis parameters, as well as for other traits which could be important for breeding.

Original languageEnglish
Pages (from-to)359-361
Number of pages3
JournalPlant Breeding
Issue number4
Publication statusPublished - Sep 1999
Externally publishedYes


  • Genetic variability
  • Helianthus annuus
  • In vitro-organogenesis
  • Inbred lines


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