Genetic control for protoplast division in recombinant inbred lines of sunflower (Helianthus annuus L)

X. Q. Huang, L. Genzbittel, M. Petitprez, A. Sarrafi

Research output: Contribution to journalArticlepeer-review

Abstract

In an attempt to elucidate the genetic variability for protoplast division parameters in sunflower, the percentage of dividing protoplasts, microcolonies and embryo-like structures were studied. Protoplasts of 32 recombinant inbred lines (RILs) of sunflower and their two parents (PAC-2 and RHA-266) were isolated from seedling hypocotyls, at density of 50,000 protoplasts/ml. The experiment was designed as a randomized complete block with 3 replications and 34 genotypes (32 RILs and two parents). The results demonstrated significant differences among the RILs and their parents (PAC-2 and RHA-266) for all parameters studied. Parental inbred line RHA-266 showed significantly higher values than PAC-2 for all the traits studied. The best RIL and the mean of 10% of the best selected RILs compared with the mean of parents presented significant differences for all the traits studied. This phenomenon might be due to the polygenic control of protoplast culture parameters and the accumulation of favourable alleles for protoplast culture traits in the RILs.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalJournal of Genetics and Breeding
Volume56
Issue number4
Publication statusPublished - Dec 2002
Externally publishedYes

Keywords

  • Genetic gain
  • Inbred line
  • Protoplast division
  • Sunflower

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