Gene cloning, expression and characterization of novel phytase from Obesumbacterium proteus

Nickolay V. Zinin, Anna V. Serkina, Mikhail S. Gelfand, Aleksei B. Shevelev, Sergei P. Sineoky

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44 Citations (Scopus)

Abstract

The gene phyA encoding phytase was isolated from Obesumbacterium proteus genomic library and sequenced. The cleavage site of the PhyA signal peptide was predicted and experimentally proved. The PhyA protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from Yersinia pestis and phytase AppA from Escherichia coli, respectively. Based on protein sequence similarity of PhyA and its homologs, the phytases form a novel subclass of the histidine acid phosphatase family. To characterize properties of the PhyA protein, we expressed the phyA gene in E. coli. The specific activity of the purified recombinant PhyA was 310 U mg-1 of protein. Recombinant PhyA showed activity at pH values from 1.5 through 6.5 with the optimum at 4.9. The temperature optimum was 40-45°C at pH 4.9. The Km value for sodium phytate was 0.34 mM with a Vmax of 435 U mg-1.

Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalFEMS Microbiology Letters
Volume236
Issue number2
DOIs
Publication statusPublished - 15 Jul 2004
Externally publishedYes

Keywords

  • AppA-like protein
  • Histidine acid phosphatase family
  • Obesumbacterium proteus
  • Phylogeny
  • Phytase

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