Future of human mitochondrial DNA editing technologies

N. Verechshagina, N. Nikitchina, Y. Yamada, Harashima, M. Tanaka, K. Orishchenko, I. Mazunin

Research output: Contribution to journalLetterpeer-review

18 Citations (Scopus)

Abstract

ATP and other metabolites, which are necessary for the development, maintenance, and functioning of bodily cells are all synthesized in the mitochondria. Multiple copies of the genome, present within the mitochondria, together with its maternal inheritance, determine the clinical manifestation and spreading of mutations in mitochondrial DNA (mtDNA). The main obstacle in the way of thorough understanding of mitochondrial biology and the development of gene therapy methods for mitochondrial diseases is the absence of systems that allow to directly change mtDNA sequence. Here, we discuss existing methods of manipulating the level of mtDNA heteroplasmy, as well as the latest systems, that could be used in the future as tools for human mitochondrial genome editing.

Original languageEnglish
Pages (from-to)214-221
Number of pages8
JournalMitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
Volume30
Issue number2
DOIs
Publication statusPublished - 17 Feb 2019
Externally publishedYes

Keywords

  • AsCpf1
  • mitoRetron
  • mitoRGEN
  • mitoTALE
  • SpCas9

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