Frequent alternative splicing of human genes

Andrey A. Mironov, James Wildon Fickett, Mikhail S. Gelfand

Research output: Contribution to journalArticlepeer-review

431 Citations (Scopus)


Alternative splicing can produce variant proteins and expression patterns as different as the products of different genes, yet the prevalence of alternative splicing has not been quantified. Here the spliced alignment algorithm was used to make a first inventory of exon-intron structures of known human genes using EST contigs from the TIGR Human Gene Index. The results on any one gene may be incomplete and will require verification, yet the overall trends are significant. Evidence of alternative splicing was shown in 35% of genes and the majority of splicing events occurred in 5' untranslated regions, suggesting wide occurrence of alternative regulation. Most of the alternative splices of coding regions generated additional protein domains rather than alternating domains.

Original languageEnglish
Pages (from-to)1288-1293
Number of pages6
JournalGenome Research
Issue number12
Publication statusPublished - Dec 1999
Externally publishedYes


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