Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry

Séverine A. Ouvry-Patat, Matthew P. Torres, Hung Hiang Quek, Craig A. Gelfand, Patrick O'Mullan, Mikkel Nissum, Gottfried K. Schroeder, Jun Han, Monica Elliott, Deanna Dryhurst, Juan Ausio, Richard Wolfenden, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

High-efficiency prefractionation of complex protein mixtures is critical for top-down proteomics, i.e., the analysis of intact proteins by MS. Free-flow electrophoresis (FFE) can be used for IEF to separate proteins within a pH gradient according to their pIs. In an FFE system, this separation is performed entirely in the liquid phase, without the need for particulate chromatographic media, gels, or membranes. Herein, we demonstrated the compatibility of IEF-FFE with ESI-Fourier transform ICR MS (ESI-FTICR-MS) for top-down experiments. We demonstrated that IEF-FFE of intact proteins were highly reproducible between FFE instruments, between laboratories, and between analyses. Applying native (0.2% hydroxypropylmethyl cellulose) IEF-FFE to an enzyme resulted in no decrease in enzyme activity; applying either native or denaturing (8 M urea) IEF-FFE to a four-protein mixture with different pIs resulted in isolation of each protein into separate fractions in a 96-well plate. After desalting, each protein was sequenced by top-down MS/MS. As an application of this technique, chicken erythrocyte histone H2A-IV and its major modified forms were enriched by IEF-FFE. Top-down analysis revealed Lys-5 to be a major acetylation site, in addition to N-terminal acetylation.

Original languageEnglish
Pages (from-to)2798-2808
Number of pages11
JournalProteomics
Volume8
Issue number14
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

Keywords

  • Acetylation
  • Free-flow electrophoresis (FFE)
  • FTICR-MS
  • Histone H2A-IV
  • Top-down proteomics

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