Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation

Maria Slyusarenko, Nadezhda Nikiforova, Elena Sidina, Inga Nazarova, Vladimir Egorov, Yuri Garmay, Anastasiia Merdalimova, Natalia Yevlampieva, Dmitry Gorin, Anastasia Malek

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.

Original languageEnglish
Article number458
Pages (from-to)1-16
Number of pages16
JournalPolymers
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Feb 2021

Keywords

  • Dextran
  • Diagnostic
  • Extracellular nanovesicles
  • Plasma
  • Polyethylene glycol
  • Two‐-phase polymer system

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