Fluorescent proteins as a toolkit for in vivo imaging

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397 Citations (Scopus)

Abstract

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, and its mutant variants, are the only fully genetically encoded fluorescent probes available and they have proved to be excellent tools for labeling living specimens. Since 1999, numerous GFP homologues have been discovered in Anthozoa, Hydrozoa and Copepoda species, demonstrating the broad evolutionary and spectral diversity of this protein family. Mutagenic studies gave rise to diversified and optimized variants of fluorescent proteins, which have never been encountered in nature. This article gives an overview of the GFP-like proteins developed to date and their most common applications to study living specimens using fluorescence microscopy.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalTrends in Biotechnology
Volume23
Issue number12
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

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