Fluorescence imaging of epigenetic genome modifications

Maria A. Moshareva, Konstantin A. Lukyanov, Lidia V. Putlyaeva

Research output: Contribution to journalShort surveypeer-review

Abstract

Epigenome contains a lot of information about cell state. Epigenetic analysis includes primarily sequence-based methods, which provide detailed data on distribution of modifications along the genome, but are poorly applicable for screenings. Specific fluorescence labeling and imaging of epigenetic modifications is an attractive complementary approach. It is currently based mainly on histone modifications study. We expect that inclusion of DNA modifications into imaging-based study would empower the method. In this review we discuss methods for fluorescence imaging of DNA modifications (mainly 5-methylcytosine). It opens an easy way to single cell analysis and high-throughput screening. Moreover, tracking epigenome changes in live cells becomes possible with genetically encoded probes.

Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume622
DOIs
Publication statusPublished - 24 Sep 2022

Fingerprint

Dive into the research topics of 'Fluorescence imaging of epigenetic genome modifications'. Together they form a unique fingerprint.

Cite this