Fluorescence imaging of actin fine structure in tumor tissues using sir-actin staining

Natalia V. Klementieva, Ludmila B. Snopova, Natalia N. Prodanets, Olga E. Furman, Varvara V. Dudenkova, Elena V. Zagaynova, Konstantin A. Lukyanov, Alexander S. Mishin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background: The rearrangement of actin cytoskeleton is being increasingly considered a marker of cancer cell activity, but the fine structure and remodeling of microfilaments within tumor tissue still remains unclear. Materials and Methods: We used the recently introduced silicon-rhodamine (SiR)-actin dye to visualize endogenous actin within tissues by confocal or total internal reflection fluorescence microscopy. We established imaging conditions for robust blinking of SiR-actin, which makes this dye applicable for super-resolution localization microscopy, as well as for an efficient background elimination. Results: We studied tumor tissue samples in two mouse models at high resolution and revealed a complex network of thick curved bundles of actin in cancer cells in tumors. This actin pattern differed strongly from that in cancer cells in vitro and in normal tissues. Conclusion: Localization microscopy with SiR-actin provides an efficient way to visualize fine actin structure in tumor tissues. It is potentially applicable to a variety of biological and clinical samples.

Original languageEnglish
Pages (from-to)5287-5294
Number of pages8
JournalAnticancer Research
Volume36
Issue number10
DOIs
Publication statusPublished - Oct 2016
Externally publishedYes

Keywords

  • Actin fine structure
  • Fluorescence imaging
  • SiR-actin
  • Super-resolution microscopy
  • Tumor tissue

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