Integrated experimental investigations of equilibrium temperature, temperature gradient and rock thermal properties were performed for a borehole drilled in Baleykinskiy licensed oil-field (Orenburg region, Russia) to provide a reliable database on rock thermal properties and heat flow for basin and petroleum system modeling. 1699 core samples were studied with a new continuous thermal logging technique and influence of elevated temperature and pressure and fluid-saturation were estimated experimentally to determine the rock thermal conductivity, thermal anisotropy coefficient and volumetric heat capacity at formation conditions. Vertical variations in equilibrium temperature gradient were determined from three temperature logging operations in the shut-in borehole. Essential vertical variations in heat flow were established that resulted in terrestrial heat flow value determined to be by 119% higher than previous regional heat flow estimations done from earlier measurements with traditional tecniques and published in heat flow maps. A continuous profile of rock thermal conductivity was obtained from correlations of the continuous thermal logging data with well-logging data and and continuous distribution of total organic carbon was calculated from the thermal core logging data. The experimental data on rock thermal properties and heat flow provided essential improvement in basin modeling reliability.