Fast and precise protein tracking using repeated reversible photoactivation

Dmitriy M. Chudakov, Tatyana V. Chepurnykh, Vsevolod V. Belousov, Sergey Lukyanov, Konstantin A. Lukyanov

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.

Original languageEnglish
Pages (from-to)1304-1310
Number of pages7
JournalTraffic
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Keywords

  • asCP
  • asFP595
  • asulCP
  • Bid
  • Photoactivatable protein
  • Protein movement tracking
  • Reversible photoactivation

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