Far-red fluorescent tag for protein labelling

Arkady F. Fradkov, Vladislav V. Verkhusha, Dmitry B. Staroverov, Maria E. Bulina, Yurii G. Yanushevich, Vladimir I. Martynov, Sergey Lukyanov, Konstantin A. Lukyanov

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)


Practical applications of green fluorescent protein ('GFP')-like fluorescent proteins (FPs) from species of the class Anthozoa (sea anemones, corals and sea pens) are strongly restricted owing to their oligomeric nature. Here we suggest a strategy to overcome this problem by the use of two covalently linked identical red FPs as non-oligomerizing fusion tags. We have applied this approach to the dimeric far-red fluorescent protein HcRed1 and have demonstrated superiority of the tandem tag in the in vivo labelling of fine cytoskeletal structures and tiny nucleoli. In addition, a possibility of effective fluorescence resonance energy transfer ('FRET') between enhanced yellow FP mutant ('EYFP') and tandem HcRed1 was demonstrated in a protease assay.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalBiochemical Journal
Issue number1
Publication statusPublished - 15 Nov 2002
Externally publishedYes


  • DsRed
  • Green fluorescent protein (GFP)
  • HcRed
  • Tandem tag


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