External accumulation LC-ESI FTICR with automated gain control

Mikhail Belov, Gordon Anderson, Sergey Rakov, Eugene Nikolaev, Rui Zhang, Eric Strittmatter, Keqi Tang, Michael Goshe, Richard Smith

Research output: Contribution to conferencePaperpeer-review


An automated gain control (AGC) with external accumulation liquid chromatography (LC)-electrospray ionization (ESI)-Fourier transforms infrared spectroscopy (FTICR) combined with independently controlled internal calibration was developed. Three approaches for data dependent adjustment of ion accumulation in the accumulation quadrupole were evaluated. The efficiency of different AGC approaches in identification of putative peptides was investigated in the course of LC separation of a 0.05 mg/ml solution of four casein proteins digested with trypsin. The adjustment of ion accumulation on the continuous ion current on the conductance limit was found to give rise to the number of putative peptides comparable with that of the conventional LC-ESI-FTIR.

Original languageEnglish
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002


ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL


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