Exhaled breath condensate analysis from intubated newborns by nano-HPLC coupled to high resolution MS

A. S. Kononikhin, N. L. Starodubtseva, V. V. Chagovets, A. Y. Ryndin, A. A. Burov, I. A. Popov, A. E. Bugrova, R. A. Dautov, A. O. Tokareva, Y. L. Podurovskaya, O. V. Ionov, V. E. Frankevich, E. N. Nikolaev, G. T. Sukhikh

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    20 Citations (SciVal)

    Abstract

    Invasiveness of examination and therapy methods is a serious problem for intensive care and nursing of premature infants. Exhaled breath condensate (EBC) is the most attractive biofluid for non-invasive methods development in neonatology for monitoring the status of intubated infants. The aim of the study was to propose an approach for EBC sampling and analysis from mechanically ventilated neonates. EBC collection system with good reproducibility of sampling was demonstrated. Discovery-based proteomic and metabolomic studies were performed using nano-HPLC coupled to high resolution MS. Label-free semi-quantitative data were compared for intubated neonates with congenital pneumonia (12 infants) and left-sided congenital diaphragmatic hernia (12 infants) in order to define disease-specific features. Totally 119 proteins and 164 metabolites were found. A number of proteins and metabolites that can act as potential biomarkers of respiratory diseases were proposed and require further validation.

    Original languageEnglish
    Pages (from-to)97-105
    Number of pages9
    JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
    Volume1047
    DOIs
    Publication statusPublished - 15 Mar 2017

    Keywords

    • Exhaled breath condensate (EBC)
    • HPLC
    • Mass spectrometry
    • Metabolomics
    • Newborns
    • Pneumonia
    • Proteomic
    • Respiratory diseases

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