Evaluation of photocytotoxicity liposomal form of furanocoumarins Sosnowsky's hogweed

Oleg A. Kulikov, Valentin P. Ageev, Ekaterina P. Brodovskaya, Vasilisa I. Shlyapkina, Pavel S. Petrov, Mikhail N. Zharkov, Denis E. Yakobson, Igor V. Maev, Gleb B. Sukhorukov, Nikolay A. Pyataev

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Sosnovsky's hogweed, Heracleum sosnowskyi has a high photosensitizing ability. Although Sosnovsky's hogweed is known as a poisonous plant, its chemical composition and phototoxicity are poorly studied. We analyzed the chemical composition of the Sosnovsky's hogweed juice that grew in natural conditions. It was found that the content of 8-methoxypsoralen in the juice is 1332.7 mg/L, and that of 5-methoxypsoralen is 34.2 mg/L. We have developed and analyzed liposomes containing furanocoumarins of Sosnovsky's hogweed juice and studied their photocytotoxicity in L929 mouse fibroblast cell culture. It was found that liposomes containing furanocoumarins of Sosnovsky's hogweed juice are more toxic for L929 cells in comparison with liposomal forms of pure substances 8-methoxypsoralen and 5-methoxypsoralen. It was found that when exposed to UV radiation at 365 nm at a dose of 22.2 J/cm2, the liposomal form of furanocoumarins Sosnovsky's hogweed is 3 times more toxic to L929 cells than in the dark. It was found that the photocytotoxic effect of liposomal furanocoumarins Sosnovsky's hogweed is a strongly stimulation of apoptosis.The data obtained suggest that the raw material of Sosnovsky's hogweed claims to be a source of furanocoumarins, and the liposomal form, given the hydrophobic properties of furanocoumarins, is very suitable for creating a phototherapeutic drug.

Original languageEnglish
Article number109880
JournalChemico-Biological Interactions
Volume357
DOIs
Publication statusPublished - 25 Apr 2022
Externally publishedYes

Keywords

  • Apoptosis
  • Furanocoumarins
  • Liposomes
  • Photocytotoxicity
  • Sosnovsky's hogweed
  • UV-Radiation

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