Estimation of phosphorylation level of amyloid-beta isolated from human blood plasma: Ultrahigh-resolution mass spectrometry

I. A. Popov, M. I. Indeikina, S. I. Pekov, N. L. Starodubtseva, A. S. Kononikhin, M. I. Nikolaeva, E. N. Kukaev, Yu I. Kostyukevich, S. A. Kozin, A. A. Makarov, E. N. Nikolaev

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Recently, amyloid-beta (Aβ) phosphorylation at position 8 has been shown to be associated with pathogenesis of Alzheimer's disease. Since the modification occurs in the key fragment of the metal-binding domain of Aβ and should seriously affect the interaction of pS8-Aβ with zinc ions, this isoform may be a potential precursor of pathogenic oligomer forms of Aβ. Hence the level of pS8-Aβ in human biological fluids (blood, urine, cerebrospinal fluid) may reflect various stages of pathogenesis of the Alzheimer's disease. The aim of the work was to develop a prototype of an analytical method for quantitative determination of the level of pS8-Aβ isoform in binary mixtures with native Aβ in order to further use it to estimate the levels of phosphorylated amyloid-beta in blood plasma samples of patients diagnosed with Alzheimer's disease.

Original languageEnglish
Pages (from-to)607-614
Number of pages8
JournalMolecular Biology
Volume48
Issue number4
DOIs
Publication statusPublished - Jul 2014
Externally publishedYes

Keywords

  • amyloid-beta
  • mass spectrometry
  • phosphorylation
  • quantitative evaluation

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