Estimating the number of HIV-specific T-cells in healthy donors using high-throughput sequencing profiles of T-cell receptor repertoires

A. V. Eliseev, A. D. Fedorova, M. Y. Lebedin, D. M. Chudakov, M. Shugay

Research output: Contribution to journalArticlepeer-review

Abstract

In-depth study of mechanisms of immune response to the human immunodeficiency virus (HIV) is critical for better understanding of how immunodeficiency develops in patients with HIV, as well as for designing effective immunotherapy strategies and vaccines against the virus. In this work we analyze sequencing profiles of T-cell receptor repertoires previously obtained from healthy donors (601 Americans and 65 Russians) to estimate the population frequency of HIV-specific naive T-cells. We demonstrate that frequencies of T-cells recognizing different HIV epitopes vary considerably across the population (F-statistic = 2007, p < 10-100, ANOVA). Although the frequency of T-lymphocytes recognizing a particular epitope does not change significantly between the individuals, it still largely depends on the presence of certain HLA alleles (p < 0.01, post-hoc Tukey's test), cytomegalovirus infection (F = 61, p = 7 × 10-15, ANOVA), and age (Pearson correlation coefficient ranging from-0.53 to-0.14 in different groups).

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalBulletin of Russian State Medical University
Volume6
Issue number5
DOIs
Publication statusPublished - 1 Sep 2017
Externally publishedYes

Keywords

  • CMV
  • High-throughput sequencing
  • HIV
  • HLA
  • T-cell receptor

Fingerprint

Dive into the research topics of 'Estimating the number of HIV-specific T-cells in healthy donors using high-throughput sequencing profiles of T-cell receptor repertoires'. Together they form a unique fingerprint.

Cite this