Entrapment of α-chymotrypsin into hollow polyelectrolyte microcapsules

Olga P. Tiourina, Alexei A. Antipov, Gleb B. Sukhorukov, Natalia I. Larionova, Yuri Lvov, Helmuth Möhwald

Research output: Contribution to journalArticlepeer-review

Abstract

Stable hollow polyelectrolyte capsules were produced by the layer-by-layer assembling of non-biodegradable polyelectrolytes - poly(allylamine) and poly(styrenesulfonate) on melamine formaldehyde microcores followed by the core decomposition at low pH. A proteolylic enzyme, α-chymotrypsin, was encapsulated into these microcapsules with high yields of up to 100%. The encapsulation procedure was based on the protein adsorption onto the capsule shells and on the pH-dependent opening and closing of capsule wall pores. The protein in the capsules retained a high activity, and thermo- and storage stability. The nanostructured polyelectrolyte shell protected the proteinase from a high molecular weight inhibitor. Such enzyme-loaded capsules can be used as microreactors for biocatalysis.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalMacromolecular Chemistry and Physics
Volume202
Issue number11
Publication statusPublished - 4 Aug 2001
Externally publishedYes

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