Stable hollow polyelectrolyte capsules were produced by the layer-by-layer assembling of non-biodegradable polyelectrolytes - poly(allylamine) and poly(styrenesulfonate) on melamine formaldehyde microcores followed by the core decomposition at low pH. A proteolylic enzyme, α-chymotrypsin, was encapsulated into these microcapsules with high yields of up to 100%. The encapsulation procedure was based on the protein adsorption onto the capsule shells and on the pH-dependent opening and closing of capsule wall pores. The protein in the capsules retained a high activity, and thermo- and storage stability. The nanostructured polyelectrolyte shell protected the proteinase from a high molecular weight inhibitor. Such enzyme-loaded capsules can be used as microreactors for biocatalysis.
|Number of pages||6|
|Journal||Macromolecular Chemistry and Physics|
|Publication status||Published - 4 Aug 2001|