Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

Nadejda G. Balabushevitch, Gleb B. Sukhorukov, Natalia A. Moroz, Dmitry V. Volodkin, Natalia I. Larionova, Edwin Donath, Helmuth Mohwald

Research output: Contribution to journalArticlepeer-review

129 Citations (Scopus)

Abstract

A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalBiotechnology and Bioengineering
Volume76
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Colloid
  • Microcapsules
  • Polyelectrolytes
  • Protein aggregates
  • Protein encapsulation
  • Protein release
  • Ultra-thin organic films

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