Electrospray ionization-fourier transform ion cyclotron mass spectrometry using ion preselection and external accumulation for ultrahigh sensitivity

Mikhail E. Belov, Evgenii N. Nikolaev, Gordon A. Anderson, Kenneth J. Auberry, Richard Harkewicz, Richard D. Smith

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

The dynamic range of Fourier transform ion cyclotron mass spectrometry (FTICR) is typically limited by the useful charge capacity of an FTICR cell (to ∼106 to 107 elementary charges) and the minimum number of ions required to produce a useful signal (∼102 elementary charges). We show that the expansion of the dynamic range by 2 orders of magnitude can be achieved by preselecting lower abundance species in a quadrupole interface to an electrospray ionization (ESI) source. Ion preselection is then followed by ion accumulation in external to the FTICR cell a linear (2-D) quadrupole trap and subsequent transfer to the region of high magnetic field for gated trapping in the FTICR cell. Two modes of ion preselection, using either the quadrupole filtering mode or rf-only dipolar excitation, were studied and mass resolutions of 30 to 100 were achieved for selective external ion accumulation of peptides and proteins with molecular weights ranging from 500 to 17,000 Da. The ability to selectively eject the most abundant species before trapping in the FTICR has enormous practical benefits for increasing the sensitivity and dynamic range of measurements.

Original languageEnglish
Pages (from-to)38-48
Number of pages11
JournalJournal of the American Society for Mass Spectrometry
Volume12
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

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