DNA detection by dye labeled oligonucleotides using surface enhanced Raman spectroscopy

Olga E. Eremina, Timofei S. Zatsepin, Valentina M. Farzan, Irina A. Veselova, Maria I. Zvereva

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Dye labeled oligonucleotides have been applied to detection of DNA with high sensitivity using surface enhanced Raman spectroscopy (SERS). Duplex formation of dye labelled oligonucleotides with DNA targets, followed by selective conjugation with silver nanoparticles, made it possible to determine the optimal distance between the SERS label and the silver surface, which was necessary for strong signal enhancement. For all Rh6G labeled oligonucleotides the limits of detection were 3 fmol dm–3, while for Cy3 labeled oligonucleotides the signal enhancement depended on the distance between Cy3 dye and silver nanoparticles.

    Original languageEnglish
    Pages (from-to)18-21
    Number of pages4
    JournalMendeleev Communications
    Volume30
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2020

    Keywords

    • DNA detection
    • dye labeled oligonucleotides
    • optical sensors
    • promoter of human telomerase catalytic subunit gene
    • silver nanoparticles
    • specific sequence
    • surface enhanced Raman spectroscopy

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