Determination of proteomic and metabolic composition of exhaled breath condensate of newborns

A. S. Kononikhin, V. V. Chagovets, N. L. Starodubtseva, A. Y. Ryndin, A. E. Bugrova, Y. I. Kostyukevich, I. A. Popov, V. E. Frankevich, O. V. Ionov, G. T. Sukhikh, E. N. Nikolaev

Research output: Contribution to journalArticlepeer-review

Abstract

Here, the possibility of proteomic and metabolomic analysis of the composition of exhaled breath condensate of neonates with respiratory support. The developed method allows non-invasive collecting sufficient amount of the material for identification of disease-specific biomarkers. Samples were collected by using a condensing device that was incorporated into the ventilation system. The collected condensate was analyzed by liquid chromatography coupled with high resolution mass spectrometry and tandem mass spectrometry. The isolated substances were identified with a use of databases for proteins and metabolites. As a result, a number of compounds that compose the exhaled breath condensate was determined and can be considered as possible biomarkers of newborn diseases or stage of development.

Original languageEnglish
Pages (from-to)540-544
Number of pages5
JournalMolekulyarnaya Biologiya
Volume50
Issue number3
DOIs
Publication statusPublished - 1 May 2016
Externally publishedYes

Keywords

  • exhaled breath condensate (EBC)
  • mass spectrometry
  • metabolomics
  • newborns
  • proteomic analysis

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