Determinants for Escherichia coli RNA polymerase assembly within the β subunit

Yi Wang, Konstantin Severinov, Nick Loizos, David Fenyö, Ewa Heyduk, Tomasz Heyduk, Brian T. Chait, Seth A. Darst

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

We used binding assays and other approaches to identify fragments of the Escherichia coli RNAP β subunit involved in the obligatory interaction with the α subunit to form the stable assembly intermediate α2β as well as in the interaction to recruit the β' subunit into the α2β sub-assembly. We show that two regions of evolutionarily conserved sequence near the C terminus of β (conserved regions H and I) are central to the assembly of RNAP and likely make subunit-subunit contacts with both α and β'.

Original languageEnglish
Pages (from-to)648-662
Number of pages15
JournalJournal of Molecular Biology
Volume270
Issue number5
DOIs
Publication statusPublished - 1 Aug 1997
Externally publishedYes

Keywords

  • E. coli RNA polymerase assembly
  • Limited proteolysis
  • Protein-protein footprinting
  • Transcription

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