Design and performance of an ESI interface for selective external ion accumulation coupled to a Fourier transform ion cyclotron mass spectrometer

M. E. Belov, E. N. Nikolaev, G. A. Anderson, H. R. Udseth, T. P. Conrads, T. D. Veenstra, C. D. Masselon, M. V. Gorshkov, R. D. Smith

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92 Citations (Scopus)

Abstract

The coupling of Fourier Transform ion cyclotron resonance mass spectrometry (FTICR MS) with electrospray ionization has advanced the analysis of large biopolymers and provided the basis for high-throughput protein characterization (e.g., for rapid "proteome" analyses). In this work, the combination of high-performance capillary liquid chromatography with FTICR mass spectrometry and external ion accumulation has been shown to increase both sensitivity and analysis duty cycle. Instrument versatility is further improved by ion preselection followed by ion accumulation in an external linear quadrupole ion trap. The interface was tested with a 3.5-T FTICR mass spectrometer and evaluated with a number of peptides and proteins whose molecular weights ranged from 500 to 66 000. A significant increase in the sensitivity, duty cycle, and dynamic range over that of the previously used accumulated trapping was achieved, exhibiting a detection limit of ∼10 zmol (∼6000 molecules) for smaller proteins such as cytochrome c. Capillary LC external accumulation interface with FTICR was successfully applied for the study of whole-proteome mouse tryptic digests.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalAnalytical Chemistry
Volume73
Issue number2
DOIs
Publication statusPublished - 15 Jan 2001
Externally publishedYes

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