Delivery Cas9 into mitochondria

K. E. Orishchenko, J. K. Sofronova, E. G. Chupakhin, E. A. Lunev, I. O. Mazunin

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Genome editing technologies became tremendously useful in laboratories around the world. ZFN and TALENs were successfully adapted for production specific doublestranded breaks in mtDNA. Recently developed more efficient and universal CRISPR\Cas9 based technology is still in the very beginning for its adaptation for mtDNA editing. In this article, we modified nuclease Cas9, protein part of functional CRISPR\Cas9 system, for specific import into mitochondria inner compartment. Adaptation of the second system component - guide RNA, for specific delivery to the mitochondria, would allow to use it for directed degradation of mtDNA containing mutations and potentially develop approaches for mtDNA editing.

Original languageEnglish
Pages (from-to)100-105
Number of pages6
JournalGenes and Cells
Volume11
Issue number2
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Mitochondria
  • mtDNA editing
  • Nuclease Cas9

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