[Data filtration for more robust alignment of chromatograms of complex peptide mixtures].

D. M. Avtonomov, I. A. Agron, A. S. Kononikhin, I. A. Popov, E. N. Nikolaev

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In carrying out proteomic researches using mass-spectrometry there often arises a need to compare experimental data with each other (e.g. control of pathology, the labeled to unlabelled samples). If for peptide identification in different experiments one uses only their exact mass measurements and the retention time in the chromatographic column, difficulties with the identification of chromatographic peaks belonging to the same substances in different chromatograms come up (retention time normalization). Due to inevitable discrepancies in chromatographic conditions of experiments (replacement of chromatographic columns, small changes in mobile phase flow rate or solvent concentration) retention times of the same peptides will diverge from experiment to experiment. In this paper we offer a reliable method for selecting peaks from mass-chromatograms corresponding to the same peptides, which can later be used for retention time normalization (either linear or any other monotone function).

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalBioorganicheskaia khimiia
Volume37
Issue number2
Publication statusPublished - Mar 2011
Externally publishedYes

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