Current trends in quantitative proteomics

Monica H. Elliott, Derek S. Smith, Carol E. Parker, Christoph Borchers

Research output: Contribution to journalReview articlepeer-review

220 Citations (Scopus)

Abstract

It was inevitable that as soon as mass spectrometrists were able to tell biologists which proteins were in their samples, the next question would be how much of these proteins were present. This has turned out to be a much more challenging question. In this review, we describe the multiple ways that mass spectrometry has attempted to address this issue, both for relative quantitation and for absolute quantitation of proteins. There is no single method that will work for every problem or for every sample. What we present here is a variety of techniques, with guidelines that we hope will assist the researcher in selecting the most appropriate technique for the particular biological problem that needs to be addressed. We need to emphasize that this is a very active area of proteomics research - new quantitative methods are continuously being introduced and some 'pitfalls' of older methods are just being discovered. However, even though there is no perfect technique - and a better technique may be developed tomorrow - valuable information on biomarkers and pathways can be obtained using these currently available methods.

Original languageEnglish
Pages (from-to)1637-1660
Number of pages24
JournalJournal of Mass Spectrometry
Volume44
Issue number12
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

Keywords

  • O
  • DIGE, N
  • ICAT
  • iMALDI
  • iTRAQ
  • Label-free
  • MRM
  • Plasma
  • Proteomics
  • Quantitation
  • SILAC
  • SISCAPA

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