Controlled release of DNA from self-degrading microcapsules

Tatiana Borodina, Elena Markvicheva, Stanislav Kunizhev, Helmuth Möhwald, Gleb B. Sukhorukov, Oliver Kreft

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)

Abstract

Self-disintegrating microcapsules were prepared by encapsulating a highly active mix of proteases (Pronase®) into biodegradable polyelectrolyte shells. Pronase was captured by micron-sized calcium carbonate particles that were subsequently embedded into onion-like shells of poly(L-arginine) and poly(L-aspartic acid). EDTA treatment was used to extract the calcium carbonate constituents from the resulting core-shell particles. As a consequence, Pronase was released into the capsule interior and started to digest the surrounding polyelectrolyte shell. Lifetimes of such self-disintegrating capsules could be successfully adjusted to seconds, hours or days by varying the amount of encapsulated Pronase. The enzyme-mediated, sustained release of encapsulated DNA is presented as a prospective application in drug delivery.

Original languageEnglish
Pages (from-to)1894-1899
Number of pages6
JournalMacromolecular Rapid Communications
Volume28
Issue number18-19
DOIs
Publication statusPublished - 18 Sep 2007
Externally publishedYes

Keywords

  • Biodegradable polymers
  • DNA
  • Drug delivery systems
  • Enzymes
  • Microencapsulation

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