Computational identification of BioR, a transcriptional regulator of biotin metabolism in Alphaproteobacteria, and of its binding signal

Dmitry A. Rodionov, Mikhail S. Gelfand

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Comparative genomic analysis was applied to identify the biotin transcriptional regulator, BioR, in most Alphaproteobacteria, and to identify its recognition signal TTATMKATAA. BioR belongs to the GntR family of transcriptional repressors. The functional assignment is supported by three lines of evidence: (1) bioR is positionally clustered with various bio genes, both for biotin biosynthesis and transport; (2) in most cases, candidate BioR-binding sites (BIOR boxes) are observed upstream of the bioR genes, suggesting autoregulation; (3) the phyletic distribution of the BIOR boxes coincides exactly with the phyletic distribution of the bioR genes, as the genomes lacking BIOR boxes do not have orthologs of bioR. Thus, in Alphaproteobacteria, BioR seems to have assumed the role of the biotin regulator that in most other bacteria is fulfilled by the dual function biotin-protein ligase BirA having the DNA-binding helix-turn-helix domain.

Original languageEnglish
Pages (from-to)102-107
Number of pages6
JournalFEMS Microbiology Letters
Volume255
Issue number1
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Keywords

  • Alphaproteobacteria
  • BioR
  • Biotin regulation

Fingerprint

Dive into the research topics of 'Computational identification of BioR, a transcriptional regulator of biotin metabolism in Alphaproteobacteria, and of its binding signal'. Together they form a unique fingerprint.

Cite this