Comparative proteomic analysis of the thermotolerant plant portulaca oleracea acclimation to combined high temperature and humidity stress

Yunqiang Yang, Jinhui Chen, Qi Liu, Cécile Ben, Christopher D. Todd, Jisen Shi, Yongping Yang, Xiangyang Hu

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Elevated temperature and humidity are major environmental factors limiting crop yield and distribution. An understanding of the mechanisms underlying plant tolerance to high temperature and humidity may facilitate the development of cultivars adaptable to warm or humid regions. Under conditions of 90% humidity and 35 °C, the thermotolerant plant Portulaca oleracea exhibits excellent photosynthetic capability and relatively little oxidative damage. To determine the proteomic response that occurs in leaves of P. oleracea following exposure to high temperature and high humidity, a proteomic approach was performed to identify protein changes. A total of 51 differentially expressed proteins were detected and characterized functionally and structurally; these identified proteins were involved in various functional categories, mainly including material and energy metabolism, the antioxidant defense responses, protein destination and storage, and transcriptional regulation. The subset of antioxidant defense-related proteins demonstrated marked increases in activity with exposure to heat and humidity, which led to lower accumulations of H 2O2 and O2- in P. oleracea compared with the thermosensitive plant Arabidopsis thaliana. The quickly accumulations of proline content and heat-shock proteins, and depleting abscisic acid (ABA) via increasing ABA-8′-hydroxylase were also found in P. oleracea under stress conditions, that resulted into greater stomata conductance and respiration rates. On the basis of these findings, we propose that P. oleracea employs multiple strategies to enhance its adaptation to high-temperature and high-humidity conditions.

Original languageEnglish
Pages (from-to)3605-3623
Number of pages19
JournalJournal of Proteome Research
Volume11
Issue number7
DOIs
Publication statusPublished - 6 Jul 2012
Externally publishedYes

Keywords

  • ABA
  • high humidity
  • high temperature
  • Portulaca oleracea
  • ROS

Fingerprint

Dive into the research topics of 'Comparative proteomic analysis of the thermotolerant plant portulaca oleracea acclimation to combined high temperature and humidity stress'. Together they form a unique fingerprint.

Cite this